Description:

Aprotinin is a reversible inhibitor of serine proteases such as trypsin (K_i = 0.06 pM), chymotrypsin (K_i = 9.5 nM), and kallikrein (K_i = 0.8 nM). It is a much weaker inhibitor of thrombin (K_i = 0.1-0.8 mM) and trypsinogen (K_i = 2 μM). Aprotinin also competitively inhibits nNOS and iNOS with K_i values of 50 and 78 μM, respectively. Aprotinin is widely used as a protein purification tool to prevent proteases present in tissue samples from degrading the protein of interest.



Uses:

A potent protease inhibitor.Aprotinin acts as a reversible serine protease blocking and a binding agent. It is widely employed as an inhibitor of trypsin. It is also used as a proteolytic inhibitor in radioimmunoassays of polypeptide hormones. It is involved in the purification of urokinase, trypsin, and chymotrypsin on immobilized aprotinin. Further, it is used in the quantification of kallikrein activity in mixtures of esterases and proteases.